Renal Regeneration Group

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Experiment Design: Experiment type: Temporal and spatial profiling of SERPINE2 expression in the developing urogenital tract. Experimental factors: Time, sex, sample The total number of hybridizations performed in the experiment: Six UGRs (3 male, 3 female). URL: http://genex.hgu.mrc.ac.uk/Emage/database/intro.html Contact information: Name: Sean Grimmond, Institution: Institute of Molecular Bioscience, City: St Lucia, Zip: 4072, Country, Australia, Mailing address: IMB, Uni of QLD, St Lucia, QLD Australia, 4072. Telephone: 61 7 33463057, Email: s.grimmond@imb.uq.edu.au. Specimens (BioMaterials) Samples origin: Mouse Urogenital Ridge: Includes metanephros, mesonephros and gonad Provider of sample: Sex: Male Developmental stage: Theiler stage 22, 13.5dpc post coitum Age: Strain: CD1 Genotype: wild type disease state: treatments: Manipulation of specimens: Specimen Preparation (wholemount or sectioned wholemount). Fixation method (4% paraformaldehyde). Embedding process for sectioned entry (cryosection) Probe or antibody information: Probe name: uh86h05 Clone name: PN1 Accession number: AI115537 Level of characterisation of probe: Partially characterised Gene symbol: SERPINE2 Gene Name: Serine (or cysteine) proteinase inhibitor, clade E, member 2 Species from which probe was made: Mouse Strain: C3H/Hej Tissue: Mixture of male and female gonads and UGRs Developmental stage/age: 11.5-12.5dpc. Staining protocols and parameters: 1 Number of detectable probes used:1 Probe type: RNA RNA type: Antisense Labelling method: Digoxigenin Visualisation method: Alkaline phosphatase + NBT/BCIP. Protocol: PMID:10888606 Imaging data and parameters: Image acquisition parameters: Detection method: 35mm film Image magnification: Image acquisition protocol: Imaging hardware: Nikon Imaging software : Photoshop Image(s) : Image Characterizations: Structural unit ontology: http://genex.hgu.mrc.ac.uk/Databases/Anatomy/new/theiler21.shtml Hybridisation intensity scale: Strong, moderate, weak, possible, not detected, not tested Hybridisation characteristic scale: Homogenous, graded, regional, spotted, single cell, not applicable, no pattern Staining intensity: Strong Staining characteristics: Homogeneous Staining description: Staining notes: Expression strongest in the sex cords of the male gonad.